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Detekce virových zoonóz v biologických vzorcích z polárních oblastí
 LIDUCHOVÁ, Tereza
This work is focus on detection of zoonotic viruses from Greenland and Svalbard and it is divided into two parts. In the first part, the detection of the Sindbis virus genomic RNA was conducted in mosquitoes samples of Ochlerotatus nigripes collected in Svalbard and Greenland by using molecular biology methods. There was no repeatedly confirmed positive result of viral RNA in the mosquitoes samples. The presence of arboviruses in polar areas can not be completely excluded. However, the prevalence is very low so other researches are needed. In the second part, the screening of samples of cloacal swabs and samples of oropharyngeal swabs from seabird Rissa tridactyla collected in Svalbard was conducted to detect Avian Influenza A virus by using RT-qPCR. The positive result was not repeatedly confirmed as well as in case of Sindbis virus.
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